ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of mobile phone 1800 MHz electromagnetic fields (EMF) on the surface markers and the functions of human dendritic cells (DC).</p><p><b>METHODS</b>Human DCs were exposed to intermittent 5 min on/10 min off EMF with specific absorption rates (SAR) 4 W/kg for 0 h, 1 h, 12 h or 24 h, respectively. FACS analysis was used to detect the positive percentage of DC surface markers including HLA-DR and co-stimulatory molecules such as CD80, CD86, CD40 and CD11c. CCK-8 kit was adopted to examine the function of allo-mixed lymphocyte reaction (allo-MLR) of DC, and enzyme linked immunosorbent assay (ELISA) to identify the levels of IL-12p70 and TNF-alpha secreted by DC.</p><p><b>RESULT</b>Compared with the sham radiation group, after exposure to the electromagnetic fields for 1 h, 12 h, or 24 h, HLA-DR, CD80,CD86 and CD40 were all declined except CD11c. The ability of DC allo-MLR in each exposure group was decreased significantly (P<0.05), especially in the 24 h exposure group. However, the secreted levels of IL-12p70 and TNF-alpha of DC in each exposure group remained no changed.</p><p><b>CONCLUSION</b>The study showed that EMF exposure could down-regulate the surface molecules and stimulation ability of human DC.</p>
Subject(s)
Humans , B7-1 Antigen , B7-2 Antigen , Allergy and Immunology , Biomarkers , CD11c Antigen , Allergy and Immunology , Cell Phone , Cells, Cultured , Dendrites , Pathology , Dendritic Cells , Metabolism , Physiology , Radiation Effects , Electromagnetic Fields , HLA-DR Antigens , Interleukin-12 , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the therapeutic effect of cationic liposome-mediated interleukin-12 gene delivery on established murine melanoma in vivo.</p><p><b>METHODS</b>The lipofectin encapsulated pCmIL-12 plasmid was given to C57BL/6 mice on the day 3,5,7,9 after inoculation of B16 melanoma cells. The tumor size, the survival time of mice and the NK cell activity were observed.</p><p><b>RESULTS</b>The pCmIL-12 plasmid coupled with cationic liposome inhibited the tumor growth and improved the survival of mice bearing established melanoma. The activity of NK cells was also enhanced after interleukin-12 gene delivery in vivo.</p><p><b>CONCLUSION</b>Cationic liposome-mediated interleukin-12 gene delivery has significantly therapeutic effects on mice melanoma in vivo.</p>
Subject(s)
Animals , Female , Mice , Cations , DNA , Therapeutic Uses , Interleukin-12 , Genetics , Therapeutic Uses , Killer Cells, Natural , Allergy and Immunology , Liposomes , Melanoma, Experimental , Pathology , Therapeutics , Mice, Inbred C57BL , Tumor Cells, CulturedABSTRACT
<p><b>OBJECTIVE</b>To establish determination methods of eotaxin mRNA and TNF-alpha mRNA expression in the lung tissue of mice.</p><p><b>METHODS</b>Eotaxin mRNA and TNF-alpha mRNA expressions were determined by semi-quantitative RT-PCR. The functional implications of eotaxin mRNA and TNF-alpha mRNA expression were examined by detecting the numbers of total leucocytes and eosinophils in bronchoalveolar lavage fluid(BALF).</p><p><b>RESULT</b>Eotaxin mRNA and TNF-alpha mRNA expression in lung tissue total numbers of leucocyte and numbers of eosinophil in BALF increased in sensitized mice compared with those in normal mice. Dexamethasone significantly but did not inhibit eotaxin mRNA and TNF-alpha mRNA expressions, and eosinophil infiltration in the lungs of the sensitized mice. A compound preparation of traditional Chinese medicine inhibited eotaxin mRNA and eosinophil infiltration, influenced TNF-alpha mRNA expression.</p><p><b>CONCLUSION</b>Increased eotaxin mRNA expression in lung tissue is associated with eosinophil infiltration in BALF, which indicates that the methods of semi-quantitative RT-PCR may be useful to the study of the mechanism of antiasthmatic medicine.</p>
Subject(s)
Animals , Female , Male , Mice , Anti-Inflammatory Agents , Pharmacology , Asthma , Drug Therapy , Metabolism , Bronchoalveolar Lavage Fluid , Cell Biology , Chemokine CCL11 , Chemokines, CC , Genetics , Dexamethasone , Pharmacology , Disease Models, Animal , Eosinophils , Physiology , Leukocyte Count , Lung , Metabolism , Mice, Inbred ICR , RNA, Messenger , Reverse Transcriptase Polymerase Chain Reaction , Tumor Necrosis Factor-alpha , GeneticsABSTRACT
OBJECTIVE: To construct a bi-cistronic co-expression plasmid for mouse interleukin-12 and to observe its expression in vitro or in vivo.METHODS: The full-length cDNA encoding p35 and p40 was cloned into eukaryotic cells expression vector pcDNA 3.1 respectively. Subsequently,the p35 expression unit was inserted into pcDNA 3.1/p40 to produce the bi-cistronic co-expression plasmid in which the p35 and p40 genes were controlled by their own CMV.The plasmid was expressed in vitro and in vivo. RESULTS: The mIL-12 in the supernatant was detected by ELISA after the pCmIL-12 was transfected into COS-7 cells. The activity of NK cells could be augmented by the supernatant in vitro and also by by intradermal delivery of pCmIL-12 in vivo. CONCLUSION: The plasmid constructed by us can express biologically active mIL-12 in vitro and in vivo.